Structure and function of poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis T1
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چکیده
منابع مشابه
Structure and function of poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis T1.
Poly(3-hydroxybutyrate) (PHB) depolymerase from Alcaligenes faecalis T1 is composed of three domains: the catalytic (C) domain, the fibronectin type III-like (F) domain, and the substrate-binding (S) domain. We constructed domain deletion, inversion, chimera, and extra-F-domain mutants and examined their enzyme activity and PHB-binding ability. In addition, we performed substitution of 214Asp a...
متن کاملProperties of poly(3-hydroxybutyrate) depolymerase from a marine bacterium, Alcaligenes faecalis AE122.
Alcaligenes faecalis AE122 that used poly(3-hydroxybutyrate) (PHB) as a sole source of carbon was newly isolated from a coastal seawater sample. The strain required seawater for growth on PHB as well as in a nutrient broth, in which seawater could be replaced by an appropriate concentration of NaCl. PHB depolymerase was purified to homogeneity from the culture supernatant of A. faecalis AE122 b...
متن کاملCloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis.
The extracellular poly(3-hydroxybutyrate) depolymerase gene from Alcaligenes faecalis T1 was cloned into Escherichia coli DH1 by using the plasmid pUC8. An A. faecalis T1 genomic library was prepared in E. coli from a partial Sau3AI digest and screened with antibody against the depolymerase. Of the 29 antibody-positive clones, 1 (pDP14), containing about 4 kilobase pairs of A. faecalis T1 DNA, ...
متن کاملEnzymatic degradation mechanism of a solution-grown poly[(R)-3-hydroxybutyrate] single crystal with an extracellular polyhydroxybutyrate depolymerase purified from Alcaligenes faecalis T1
We report the enzymatic degradation mechanism of a solution-grown poly[(R)-3-hydroxybutyrate] (P(3HB)) single crystal with an extracellular polyhydroxybutyrate depolymerase purified from Alcaligenes faecalis T1 (PhaZAfa). The P(3HB) single crystals adsorbed on highly ordered pyrolytic graphite were incubated with a wild-type or a hydrolytic-activity-disrupted mutant of PhaZAfa, and morphologica...
متن کاملBiocatalytic Synthesis of 4-Hydroxyvaleric Acid from Levulinic Acid by Engineered 3-Hydroxybutyrate Dehydrogenase from Alcaligenes faecalis
Enzymatic conversion of levulinic acid (LA) to 4-hydroxyvaleric acid (4HV) was performed by an engineered oxidoreductase. LA, the reactant of this study, is a versatile chemical building block produced from cellulosic biomass, and can be converted into valuable chemicals and fuels. 4HV, the product of this study, can be used as a monomer of bio-polyester and a precursor of bio-fuels. Since ther...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1997
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.179.22.6965-6970.1997